Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 394, Issue 1-2, Pages 80-87Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2014.06.018
Keywords
Obesity; Adipocyte; Transcriptome analysis; Network modeling; NOD-like receptor pathway; Inflammasome
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Funding
- MacDonald Foundation
- NIH [R24DK087723, R01CA121225, R01AG028928]
- JS Dunn Research Foundation
- TT & WF Chao Foundation
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Adipose tissue inflammation increases with obesity, but adipocyte vs. immune cell contributions are unclear. In the present study, transcriptome analyses were performed on highly-purified subcutaneous adipocytes from lean and obese women, and differentially expressed genes/pathways were determined in both adipocyte and stromal vascular fraction (SVF) samples. Adipocyte but not SVF expression of NOD-like receptor pathway genes, including NLRP3 and PYCARD, which regulate caspase-1-mediated IL-1 beta secretion, correlated with adiposity phenotypes and adipocyte class II major histocompatibility complex (MHCII) gene expression, but only MHCII remained after adjusting for age and body mass index. IFN gamma stimulated adipocyte MHCII, NLRP3 and caspase-1 expression, while adipocyte MHCII-mediated CD4+ T cell activation, an important factor in adipose inflammation, induced IFN gamma-dependent adipocyte IL-1 beta secretion. These results uncover a dialogue regulated by interactions among T cell IFN gamma and adipocyte MHCII and NLRP3 inflammasome activity that appears to initiate and escalate adipose tissue inflammation during obesity. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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