Journal
SCIENCE
Volume 348, Issue 6233, Pages 442-444Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaa5945
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Funding
- NIH [R01 GM067247, R56 NS029870]
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An organism with a single recessive loss-of-function allele will typically have a wild-type phenotype, whereas individuals homozygous for two copies of the allele will display a mutant phenotype. We have developed a method called the mutagenic chain reaction (MCR), which is based on the CRISPR/Cas9 genome-editing system for generating autocatalytic mutations, to produce homozygous loss-of-function mutations. In Drosophila, we found that MCR mutations efficiently spread from their chromosome of origin to the homologous chromosome, thereby converting heterozygous mutations to homozygosity in the vast majority of somatic and germline cells. MCR technology should have broad applications in diverse organisms.
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