Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 350, Issue 1, Pages 39-52Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2011.11.019
Keywords
Transforming growth factor-beta; Follistatin; Myostatin; Myosin heavy chain II
Categories
Funding
- National Institute of Aging [SC1AG033407]
- Charles Drew University MSI [5S21MD000103]
- [1SC1CA165865-01A1]
- [5U54CA 143931]
Ask authors/readers for more resources
Testosterone (T) administration is associated with increased satellite cell number and skeletal muscle hypertrophy, although there is considerable heterogeneity in the response of different skeletal muscle groups to T in vivo. We investigated the effects of T on the growth and differentiation of satellite cells isolated from levator ani (LA) and gastrocnemius (gastroc) muscles. T up regulated follistatin (Fst) expression, but down regulated the mRNA and protein expression of a number of genes in the transforming growth factor-beta (TGF-beta)-signaling pathway. Inhibition of Fst expression by small interfering RNA (siRNA) inhibited myogenic differentiation and blocked the pro-myogenic effects of T. Treatment of satellite cells with T or Fst up regulated the expression of Pax7 and PCNA, and increased their proliferation. T and Fst blocked TGF-beta induced inhibition of growth and myogenic differentiation and down regulated TGF-beta-dependent transcriptome in both LA and gastroc cells. We conclude that T stimulation of satellite cell proliferation and myogenic differentiation are associated with up regulation of Fst and inhibition of TGF-beta-signaling. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available