Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 345, Issue 1-2, Pages 38-47Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2011.07.006
Keywords
c-kit mRNA; KL; In vitro culture; Caprine; Ovary
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Funding
- National Council for Scientific and Technological Development (CNPq, Brazil) [554812/2006-1- RENORBIO]
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- Brazilian Innovation Agency (FINEP)
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This study evaluated the levels of c-kit mRNA in goat follicles and the effects of kit ligand (KL) on the in vitro development of cultured preantral follicles. Preantral follicles isolated from goat ovarian cortex were cultured for 18 days in alpha-MEM(+) supplemented with KL (0, 50 or 100 ng/mL) in the absence or presence of follicle stimulating hormone (FSH). Real-time PCR showed that c-kit mRNA was higher in primordial and primary follicles than in secondary stage. Regarding the culture, KL addition in the absence of FSH improved the follicular survival, antrum formation, oocyte growth and meiotic resumption. KL-positive effects were not observed in the presence of FSH. In conclusion, c-kit mRNAs are detected in all follicular categories. KL promotes the survival and antral cavity formation of caprine preantral follicles after in vitro culture, as well as the growth and meiotic resumption of their oocytes in the absence of FSH. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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