Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 330, Issue 1-2, Pages 49-58Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2010.08.007
Keywords
Adenosine; AMPK; Lactate; ZO-1; Sertoli cell
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Funding
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT 25365, PICT 1004]
- CONICET, Argentina [PIP 5479, PIP 806]
- CONICET
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This work evaluates adenosine effects on Sertoli cell functions, which are different to those resulting from occupancy of purinergic receptors. The effects of adenosine and N-6-cyclohexyladenosine (CHA) - an A(1) receptor agonist resistant to cellular uptake - on Sertoli cell physiology were compared. Adenosine but not CHA increased lactate production, glucose uptake, GLUT1. LDHA and MCT4 mRNA levels, and stabilized ZO-1 protein at the cell membrane. These differential effects suggested a mechanism of action of adenosine that cannot be solely explained by occupancy of type A(1) purinergic receptors. Activation by adenosine but not by CHA of AMPK was observed. AMPK participation in lactate production and ZO-1 stabilization was confirmed by utilizing specific inhibitors. Altogether, these results suggest that activation of AMPK by adenosine promotes lactate offer to germ cells and cooperates in the maintenance of junctional complex integrity, thus contributing to the preservation of an optimum microenvironment for a successful spermatogenesis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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