Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 292, Issue 1-2, Pages 20-25Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2008.05.002
Keywords
adiponectin; granulocyte colony-stimulating factor; macrophage
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [19390541]
- Grants-in-Aid for Scientific Research [19390541] Funding Source: KAKEN
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Adiponectin, an adipocyte-derived cytokine, affects a number of physiological processes, including immune function and inflammation. We investigated whether globular adiponectin (gAd) affects the expression of inflammation-related genes in murine macrophages (RAW264 cells). DNA microarray analysis indicated that granulocyte colony-stimulating factor (G-CSF) showed the largest increase in expression in gAd-stimulated RAW264 cells. The gAd-induced secretion of G-CSF increased in a time- and close-dependent manner. U0126 (MEK1/2 inhibitor) and PD98059 (MEK1 inhibitor) reduced the gAd-induced G-CSF mRNA expression and G-CSF Protein production. gAd induced the phosphorylation of MEK1/2 and ERK1/2 in RAW264 cells. In addition, the gAd-induced phosphorylation of MEK1/2 and ERK1/2 was dramatically reduced by PD98059 and U0126, respectively. Collectively, these results suggest that MEK1/2-ERK1/2 signaling is involved in the adiponectin-induced secretion of G-CSF. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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