Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 30, Issue 17, Pages 4254-4266Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00142-10
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Funding
- Spain and Hungary [HH2006-0025]
- Hungary and France [FR-33/08]
- Hungarian State Science Fund [OTKA PD72491, OTKA K77443]
- FRM
- European Community [HPRN-CT 00504228, STREP LSHG-CT-2004-502950, EUTRACC LSHG-CT-2007-037445]
- Reseau National des Genopoles [260]
- INCA [2008-UBICAN]
- Spanish Ministry of Research [BFU2006-10180]
- TAF-CHROMATIN European Network [MRTN-CT-2004-504288]
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Uncovering mechanisms that regulate ecdysone production is an important step toward understanding the regulation of insect metamorphosis and processes in steroid-related pathologies. We report here the transcriptome analysis of Drosophila melanogaster dAda2a and dAda3 mutants, in which subunits of the ATAC acetyltransferase complex are affected. In agreement with the fact that these mutations lead to lethality at the start of metamorphosis, both the ecdysone levels and the ecdysone receptor binding to polytene chromosomes are reduced in these flies. The cytochrome genes (spookier, phantom, disembodied, and shadow) involved in steroid conversion in the ring gland are downregulated, while the gene shade, which is involved in converting ecdysone into its active form in the periphery, is upregulated in these dATAC subunit mutants. Moreover, driven expression of dAda3 at the site of ecdysone synthesis partially rescues dAda3 mutants. Mutants of dAda2b, a subunit of the dSAGA histone acetyltransferase complex, do not share phenotype characteristics and RNA profile alterations with dAda2a mutants, indicating that the ecdysone biosynthesis genes are regulated by dATAC, but not by dSAGA. Thus, we provide one of the first examples of the coordinated regulation of a functionally linked set of genes by the metazoan-specific ATAC complex.
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