Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 30, Issue 1, Pages 245-259Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00983-09
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Funding
- NIH-RO1 [GM071775A]
- MDA Research Grant
- NIH NRSA [1F31-GM081975]
- AHA fellowship [0815083E]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [F31GM081975] Funding Source: NIH RePORTER
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The intricate biogenesis of multimeric organellar enzymes of dual genetic origin entails several levels of regulation. In Saccharomyces cerevisiae, mitochondrial cytochrome c oxidase (COX) assembly is regulated translationally. Synthesis of subunit 1 (Cox1) is contingent on the availability of its assembly partners, thereby acting as a negative feedback loop that coordinates COX1 mRNA translation with Cox1 utilization during COX assembly. The COX1 mRNA-specific translational activator Mss51 plays a fundamental role in this process. Here, we report that Mss51 successively interacts with the COX1 mRNA translational apparatus, newly synthesized Cox1, and other COX assembly factors during Cox1 maturation/assembly. Notably, the mitochondrial Hsp70 chaperone Ssc1 is shown to be an Mss51 partner throughout its metabolic cycle. We conclude that Ssc1, by interacting with Mss51 and Mss51-containing complexes, plays a critical role in Cox1 biogenesis, COX assembly, and the translational regulation of these processes.
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