Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 28, Issue 5, Pages 1724-1738Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.01717-07
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Funding
- NCI NIH HHS [T32 CA009370, CA14195, CA80100, CA009370, P30 CA014195, R01 CA080100] Funding Source: Medline
- NIGMS NIH HHS [R01 GM059321-08, R01 GM059321, GM059321] Funding Source: Medline
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The minichromosome maintenance (MCM) complex plays essential, conserved roles throughout DNA synthesis: first, as a component of the prereplication complex at origins and, then, as a helicase associated with replication forks. Here we use fission yeast (Schizosaccharomyces pombe) as a model to demonstrate a role for the MCM complex in protecting replication fork structure and promoting recovery from replication arrest. Loss of MCM function generates lethal double-strand breaks at sites of DNA synthesis during replication elongation, suggesting replication fork collapse. MCM function also maintains the stability of forks stalled by hydroxyurea that activate the replication checkpoint. In cells where the checkpoint is activated, Mcm4 binds the Cds1 kinase and undergoes Cds1-dependent phosphorylation. MCM proteins also interact with proteins involved in homologous recombination, which promotes recovery from arrest by ensuring normal mitosis. We suggest that the MCM complex links replication fork stabilization with checkpoint arrest and recovery through direct interactions with checkpoint and recombination proteins and that this role in S-phase genome stability is conserved from yeast to human cells.
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