Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 28, Issue 22, Pages 6731-6745Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.02103-07
Keywords
-
Categories
Funding
- Federation des Groupements des Entreprises Francaises dans la Lutte contre le Cancer
- Comites du Nord et de l'Aisne de la Ligue Nationale contre le Cancer
- Association pour la Recherche sur le Cancer (ARC)
- Ministere de l'Education Nationale et de la Recherche
- ARC [4868]
- Institut National du Cancer
- GIS Longevite [GISLO401]
- Fond National de la Science (ACI Jeune Chercheur)
- Agence Nationale de La Recherche [ANR-07-BLAN-0052-02]
- Centre National de la Recherche Scientifique
- Agence Nationale de la Recherche (ANR) [ANR-07-BLAN-0052] Funding Source: Agence Nationale de la Recherche (ANR)
Ask authors/readers for more resources
The H19/IGFf2 locus belongs to a large imprinted domain located on human chromosome 11p15.5 ( homologue to mouse distal chromosome 7). The H19 gene is expressed from the maternal allele, while IGF2 is paternally expressed. Natural antisense transcripts and intergenic transcription have been involved in many aspects of eukaryotic gene expression, including genomic imprinting and RNA interference. However, apart from the identification of some IGF2 antisense transcripts, few data are available on that topic at the H19/IGF2 locus. We identify here a novel transcriptional activity at both the human and the mouse H19/IGF2 imprinted loci. This activity occurs antisense to the H19 gene and has the potential to produce a single 120-kb transcript that we called the 91H RNA. This nuclear and short-lived RNA is not imprinted in mouse but is expressed predominantly from the maternal allele in both mice and humans within the H19 gene region. Moreover, the transcript is stabilized in breast cancer cells and overexpressed in human breast tumors. Finally, knockdown experiments showed that, in humans, 91H, rather than affecting H19 expression, regulates IGF2 expression in trans.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available