Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 394, Issue 1-2, Pages 307-314Publisher
SPRINGER
DOI: 10.1007/s11010-014-2098-z
Keywords
Protein-protein interaction; beta-oxidation; Carnitine; Mitochondria; Channeling
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Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 are members of the carnitine system, which are responsible of the regulation of the mitochondrial CoA/acyl-CoA ratio and of supplying substrates for the -oxidation to mitochondria. This study, using cross-Linking reagent, Blue native electrophoresis and immunoprecipitation followed by detection with immunoblotting, shows conclusive evidence about the interaction between carnitine palmitoyltransferase 2 and carnitine/acylcarnitine translocase supporting the channeling of acylcarnitines and carnitine at level of the inner mitochondrial membrane.
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