Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 394, Issue 1-2, Pages 225-235Publisher
SPRINGER
DOI: 10.1007/s11010-014-2099-y
Keywords
Apoptosis; Breast cancer; Doxorubicin; Drug combination; Mistletoe lectin
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Funding
- Establishment of Economic Technology for the Cultivation of Mistletoe and Development of Strategic Export Products Using Its Functional Compounds, Ministry of Agriculture, Food and Rural Affairs, South Korea [313011-3]
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We studied the effects, either combined or alone, of lectin from Korean mistletoe (Viscum album var. coloratum agglutinin, VCA) and doxorubicin (DOX) in MCF-7 (estrogen receptor-positive) and MDA-MB231 (estrogen receptor-negative) human breast cancer cells. When VCA and DOX were combined, a strong synergistic effect was shown in cell growth inhibition, compared to VCA or DOX treatment alone. In quantitative apoptosis studies analyzed by flow cytometry, a combination of two agents showed an increase in apoptosis in both cells, compared to agents alone. Also, pro-apoptotic proteins including Bax, Bik, and Puma were increased in both cells, and the survival factor Bcl-2 was inhibited in MCF-7 cells when drugs were combined. Furthermore, VCA combined with DOX mediated S phase arrest, accompanied with a decrease of cell number at G0/G1 phase. This suggests that VCA and DOX combination may possibly lead to a novel strategy for the treatment of breast cancer.
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