Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 394, Issue 1-2, Pages 275-281Publisher
SPRINGER
DOI: 10.1007/s11010-014-2103-6
Keywords
miR-506; CD151; PRRSV; MARC-145; Porcine
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Funding
- National Science and Technology Support Project [2011BAD28B01]
- National Pig Industry Technology System [CARS-36]
- Hubei Agricultural Science and Technology Innovation Center [2011-620-001-003]
- Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding [2013ZD101]
- Hubei Province Public Welfare Scientific Research Project [2013BBB15]
- Youth Foundation of Hubei Provincial Academy of Agricultural Sciences [2013NKYJJ11]
- Hubei Introducing Training Programme Foundation for the Top Talents
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Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important diseases of swine, which is caused by PRRS virus (PRRSV). CD151, one of PRRSV entry mediators, determines the cell susceptibility for PRRSV. Emerging evidence indicates that the host microRNAs (miRNAs) play key roles in modulating virus infection and viral pathogenesis. In the present study, targeting porcine CD151 miRNAs were identified, and their function during PRRSV infection in MARC-145 cells was further verified. We found that miR-506 could directly target porcine CD151 3'-UTR mRNA by luciferase reporter assay. Overexpression of miR-506 significantly decreased CD151 expression at both mRNA and protein levels. Furthermore, overexpression of miR-506 reduced cellular PRRSV replication and virus release in MARC-145 cells. Our results suggested that miR-506 could inhibit PRRSV replication by directly targeting PRRSV receptor of CD151 in MARC-145 cells. However, the molecular mechanisms of miR-506 and its function in vivo need further investigation.
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