Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 393, Issue 1-2, Pages 301-307Publisher
SPRINGER
DOI: 10.1007/s11010-014-2074-7
Keywords
Glioma; Biomarker; Gene expression; Heat shock protein 70 (HSP70/HSPA)
Categories
Funding
- University of Central Lancashire
- Sydney Driscoll Neuroscience Foundation (SDNF)
Ask authors/readers for more resources
Production of heat shock protein 70 (HSP70/HSPA) is induced by a wide range of cellular stress conditions, such as cancer and hypoxia, with production also being linked to tumourigenesis. HSPA mRNA transcripts and proteins were examined in three human glioma cell lines, representing astrocytoma, oligodendroglioma and glioblastoma, plus 18 clinical brain tissue samples. GAPDH was used as a control gene throughout these studies and exhibited a consistent level of expression in a normal astrocyte cell line, tumourous cell lines and tissue samples. In contrast, the average HSPA mRNA copy numbers detected in glioblastoma tissue were between 1.8- and 8.8-fold higher than in lower grade glioma and control tissue, respectively, which is suggestive of a grade-related transcription profile. Similar patterns of grade-related expression were also observed in glioma cell lines. This study indicates for the first time that HSPA expression in glioma cells may possibly be grade related, and hence could have potential as a prognostic marker.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available