Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 378, Issue 1-2, Pages 19-28Publisher
SPRINGER
DOI: 10.1007/s11010-013-1589-7
Keywords
Continuous mechanical strain; Bone mesenchymal stem cell; Osteogenesis; P38-Runx2
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Funding
- National Natural Science Foundation of China [30901698, 10972142]
- Collaborative Foundation of Medical and Engineering Science of Shanghai Jiaotong University [YG2012MS40]
- Key Basic Research Foundation of the Shanghai Committee of Science and Technology, China [12JC1405700]
- Innovative Research Team of Shanghai Municipal Education Commission
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Mechanical stimuli play a significant role in the regulation of bone remodeling during orthodontic tooth movement. However, the correlation between mechanical strain and bone remodeling is still poorly understood. In this study, we used a model of continuous mechanical strain (CMS) on bone mesenchymal stem cells (BMSCs) to investigate the proliferation and osteogenic differentiation of BMSCs and the mechanism of mechano-transduction. A CMS of 10 % at 1 Hz suppressed the proliferation of BMSCs and induced early osteogenic differentiation within 48 h by activating Runx2 and increasing alkaline phosphatase (ALP) activity and mRNA expression of osteogenesis-related genes (ALP, collagen type I, and osteopontin). Regarding mitogen-activated protein kinase (MAPK) activation, CMS induced phased phosphorylation of p38 consisting of a rapid induction of p38 MAPK at 10 min and a rapid decay after 1 h. Furthermore, the potent p38 inhibitor SB203580 blocked the induction of p38 MAPK signaling, but had little effect on subsequent osteogenic events. These results demonstrate that mechanical strain may act as a stimulator to induce the differentiation of BMSCs into osteoblasts, which is a vital function for bone formation in orthodontic tooth movement. However, activation of the p38 signaling pathway may not be involved in this process.
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