Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 365, Issue 1-2, Pages 29-35Publisher
SPRINGER
DOI: 10.1007/s11010-012-1240-z
Keywords
Curcumin; Resveratrol; FANCF; Promoter hypomethylation; Cervical cancer
Categories
Funding
- Council of Scientific and Industrial Research (CSIR), New Delhi, India
Ask authors/readers for more resources
Curcumin and resveratrol were evaluated for their potential to cause reversal of promoter hypermethylation and associated gene expression of FANCF in SiHa cell line. Methylation specific PCR along with bisulphite sequencing revealed the demethylation of 12 CpG sites out of 15 CpG sites spanning +280 to +432 region of FANCF promoter after treatment with curcumin and fivefold up regulation of FANCF gene expression as shown by qRT-PCR. In vitro methylation assay also showed that M.SssI an analogue of DNMT1 was effectively inhibited at 50 mu M concentration of curcumin. Resveratrol was not found to be effective in causing reversal of promoter hypermethylation of FANCF gene when used at 20 mu M for 4 days in SiHa cell line.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available