Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 373, Issue 1-2, Pages 19-28Publisher
SPRINGER
DOI: 10.1007/s11010-012-1471-z
Keywords
Atrazine; Quercetin; Nuclear factor-kappaB; Interstitial Leydig cells; Oxidative stress; Steroidogenic enzymes
Categories
Ask authors/readers for more resources
Quercetin has been reported to protect testicular cells from oxidative damage induced by environmental chemicals. In this study, we isolated interstitial Leydig cells (ILCs) from immature rats, set-up ILCs culture, co-treated cells with atrazine (ATZ) and quercetin (QT), evaluated toxicity, and measured the expression levels of antioxidant enzymes and nuclear factor-kappaB (NF-kappa B) and levels of steroidogenic enzymes. ATZ decreased ILCs viability at concentrations higher than 10 mu g/mL and increased reactive oxygen species, malondialdehyde (MDA), and glutathione levels. ATZ also increased glutathione peroxidase, glutathione reductase, and glutathione-S-transferase and decreased superoxide dismutase-1 (sod1) and superoxide dismutase-2 (sod2) messenger RNA (mRNA) levels which were prevented by QT. The changes in the MDA levels and lactate dehydrogenase leakage induced by ATZ (50 mu g/mL) were also prevented on co-treatment with QT (50 mu M). Furthermore, ATZ-induced 3 beta- and 17 beta-hydroxysteroid dehydrogenase activities and NF-kappa B-expressions at the mRNA and protein levels were also recovered to control value on co-treatment with QT. These data showed that QT protected against ATZ-induced ILCs toxicity by restoring the expression of NF-kappa B and steroidogenic activity and by preventing the oxidative stress.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available