Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 367, Issue 1-2, Pages 195-203Publisher
SPRINGER
DOI: 10.1007/s11010-012-1333-8
Keywords
Twist; Epithelial-mesenchymal transition; Gastric cancer; RNA interference
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Funding
- Youth Training Plan of Sun Yat-Sen University [10ykpy38]
- Research Award Fund for Outstanding Young researchers in Sun Yat-sen Cancer Center [303045172006, 303045172005]
- National Natural Science Foundation of China [30901728]
- Science & Technology Pillar Program of Guangdong Province [2011B031800220]
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This study aimed to investigate the expression of Twist in gastric cancer tissues and its correlation between Twist and the epithelial-mesenchymal transition (EMT). By means of RT-PCR and Western blot, the mRNA and protein expressions of Twist, E-cadherin, and Vimentin in 61 gastric cancer tissues and adjacent normal tissues were detected. The positive rates of Twist, E-cadherin, and Vimentin mRNA expression in gastric cancer tissues were 73.9. 40.6, and 60.9 %, respectively; compared to the expression of these genes in adjacent normal tissues (2.9, 75.4, and 27.5 %), the differences were significant (p < 0.05). The E-cadherin protein expression level in gastric cancer tissues was significantly lower than that in the adjacent normal tissues (p < 0.05). After the transfection of Twist siRNA into the MKN45 cells, the protein expression of Twist was significantly reduced (p < 0.05), the protein expression of E-cadherin was significantly increased, and the number of cells that passed through the Transwell chamber was significantly lower than that in the non-transfected control group as well as the transfected control group (p < 0.05). Twist may be associated with the epithelial-mesenchymal transition in gastric cancer and the tumorigenesis, invasion, and metastasis of gastric cancer.
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