Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 363, Issue 1-2, Pages 11-19Publisher
SPRINGER
DOI: 10.1007/s11010-011-1153-2
Keywords
U937; Foam cell formation; ox-LDL; Vitamin E; NF-kappa B pathway
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The role of antioxidant supplementation with vitamin E in the prevention of atherosclerosis has been a topic of considerable recent interest. The relevance of vitamin E for macrophage-derived foam cell formation, a hallmark of atherosclerosis, however, has not been unequivocally resolved. Here, we investigated the effect of Oxidized LDL (ox-LDL) and vitamin E on lipid accumulation and total cholesterol content in U937 macrophages, reactive oxygen species generation and expression of nuclear factor-kappa B (NF-kappa B) signaling pathway. The results showed that the mRNA expression and protein levels of P-selectin were evident in U937 macrophages treated with ox-LDL and vitamin E, which indicating that expression of P-selectin is important in macrophage-derived foam cell formation. Moreover, P-selectin changes in ox-LDL-induced foam cell formation can be mediated by vitamin E through activities of nuclear NF-kappa B activated by serine phosphorylation of NF-kappa B inhibitor alpha, suggesting that activation of NF-kappa B pathway by macrophages may occur. Taken together, these data suggested that vitamin E can prevent ox-LDL-induced foam cell macrophages formation through modulating the activities of oxidative stress-induced NF-kappa B pathway.
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