Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 353, Issue 1-2, Pages 167-176Publisher
SPRINGER
DOI: 10.1007/s11010-011-0783-8
Keywords
Melatonin; Hydrogen peroxide; Apoptosis; Leukaemia cells
Categories
Funding
- Junta de Extremadura [PRI07-A024]
- Ministerio de Ciencia e Innovacion-Fondo Europeo de Desarrollo Regional [BFU2007-60091, BFU2010-15049]
- Consejeria de Economia, Comercio e Innovacion-Fondo Social Europeo [PRE06070, REI09009]
- Ministerio de Ciencia e Innovacion [AP2009-0753]
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Melatonin is an indoleamine secreted by the pineal gland that shows multiple tasks. This ubiquitously acting free radical scavenger has recently been shown to stimulate the production of reactive oxygen species (ROS) in tumour cells, making them undergo apoptosis, whilst it prevents apoptosis in healthy cells. The mechanisms by which melatonin exerts these dual actions are, however, not yet clearly understood. Thus, the aim of this study was to further investigate how melatonin can enhance oxidative stress-induced apoptosis in a leukaemia cell line. The results show that melatonin increased the apoptotic effects of H(2)O(2) in human myeloid HL-60 cells as assessed by cellular viability, mitochondrial permeability transition induction, mitochondrial membrane depolarization, ROS generation, caspases 3, 8 and 9 activity, phosphatidylserine externalization, and DNA fragmentation techniques. When healthy leucocytes were exposed to H(2)O(2), melatonin increased the viability of the cells. Taken together, the findings indicate that melatonin is a potential physiological tool capable of protecting healthy cells from chemotherapy-induced ROS production as well as inducing tumour cell death. Because cancer cells manifest increased oxidative stress as a result of their elevated metabolism, the use of melatonin may be useful in impairing their ROS buffering capacity.
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