Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 340, Issue 1-2, Pages 125-131Publisher
SPRINGER
DOI: 10.1007/s11010-010-0409-6
Keywords
Ceramide; Sphingomyelin; Liver; Nuclei; High fat diet; Sphingomyelinase
Categories
Funding
- Medical University of Bialystok [3-18787, 3-18717]
Ask authors/readers for more resources
Obesity increases the risk for hepatic steatosis. Recent studies have demonstrated that high fat diet (HFD) may affect sphingolipid formation in skeletal muscles, heart, and other tissues. In this work we sought to investigate whether HFD feeding provokes changes in content and fatty acids (FAs) composition of sphingomyelin and ceramide at the level of liver and hepatic nuclei. Furthermore, we investigated whether the ceramide formation is related to the activity of either neutral sphingomyelinase (N-SMase) or acidic sphingomyelinase (A-SMase). Three weeks of HFD provision induced pronounced ceramide and sphingomyelin accumulation in both liver and hepatic nuclei, accompanied by increased activity of N-SMase but not A-SMase. Furthermore, a shift toward greater FAs saturation status in these sphingolipids was also observed. These findings support the conclusion that HFD has a major impact on sphingolipid metabolism not only in the liver, but also in hepatic nuclei.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available