Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 340, Issue 1-2, Pages 161-167Publisher
SPRINGER
DOI: 10.1007/s11010-010-0413-x
Keywords
Oncology database; Shank-associated RH domain-interacting protein; Post-synaptic density; Immunohistochemistry; Oncogene; Invasion assay
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Funding
- National Research Foundation [FG09-21-16, 2009-0067522]
- Korean Government [KRF-2006-331-C00268]
- National Research Foundation of Korea [2009-0067522] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In order to discover previously unidentified cancer-associated genes, we analyzed genome-wide differences in gene expression between tumor biopsies and normal tissues. Among those differentially regulated genes, we identified Sharpin (Shank-associated RH domain-interacting protein) as a commonly up-regulated gene in multiple human cancer types. Although rat Sharpin is reported to interact with Shank1, a multidomain scaffold protein localized in postsynaptic densities, its exact roles are unknown. Whereas human Sharpin homologue was primarily localized in the cytosol of cultured cells, they were detected in both cytosol and nucleus of the cells from ovarian and liver cancer tissues using immunohistochemical staining. In addition, Chinese ovary hamster cells over-expressing Sharpin exhibited enhanced cancer-specific phenotypes in multiple in vitro tumor assays. Taken together, the results suggest that Sharpin is not an inert scaffold protein, but may play tumor-associated roles during cancer biogenesis.
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