Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 333, Issue 1-2, Pages 65-72Publisher
SPRINGER
DOI: 10.1007/s11010-009-0205-3
Keywords
Cathepsin B; D-GalN/TNF-alpha; Lung injury; Oxidative stress; TRAF-1
Categories
Funding
- Istanbul University [UDP/ 2602, ACIP/1790]
Ask authors/readers for more resources
The present study was designed to investigate the effects of benzyloxicarbonyl-l-phenylalanyl-alanine-fluoromethylketone (Z-FA.FMK), an inhibitor of cathepsin B on lung injury that occurs concurrently with liver injury induced by d-galactosamine/tumor necrosis factor-alpha (d-GalN/TNF-alpha). Four groups of BALB/c male mice were treated as follows: Group 1-mice receiving intravenous (iv) injections of physiological saline; Group 2-administered with 8 mg/kg Z-FA.FMK by iv injection; Group 3-mice treated with 700 mg/kg d-GalN and 15 mu g/kg TNF-alpha by sequential intraperitoneal (ip) injection; Group 4-treated with 700 mg/kg d-GalN and 15 mu g/kg TNF-alpha by sequential ip injection 1 h after administration with 8 mg/kg Z-FA.FMK. Mice from Groups 3 and 4 were sacrificed 4 h after d-GalN/TNF-alpha injections. The mice treated with d-GalN/TNF-alpha showed lung damage; increased TNF receptor-associated factor immunoreactivity, lipid peroxidation, protein carbonyl content, and lactate dehydrogenase activity; decreased catalase, superoxide dismutase, and paraoxonase activities. Treatment with Z-FA.FMK resulted in an improvement of these alterations in d-GalN/TNF-alpha-administered mice. The apoptotic index of type-II pneumocytes was the almost same in the four study groups, but pneumocytes labeled with proliferating cell nuclear antigen antibody was more numerous in Group 4 mice. Our results show that d-GalN/TNF-alpha results in lung damage without induction of apoptosis. Treatment with Z-FA.FMK stimulates proliferation of type-II pneumocytes and improves degenerative alterations in injured lung occurred with liver injury induced by d-GalN/TNF-alpha.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available