Gene targeting of CK2 catalytic subunits

Protein kinase CK2 is a highly conserved and ubiquitous serine-threonine kinase. It is a tetrameric enzyme that is made up of two regulatory CK2 beta subunits and two catalytic subunits, either CK2 alpha/CK2 alpha, CK2 alpha/CK2 alpha', or CK2 alpha'/CK2 alpha'. Although the two catalytic subunits diverge in their C termini, their enzymatic activities are similar. To identify the specific function of the two catalytic subunits in development, we have deleted them individually from the mouse genome by homologous recombination. We have previously reported that CK2 alpha' is essential for male germ cell development, and we now demonstrate that CK2 alpha has an essential role in embryogenesis, as mice lacking CK2 alpha die in mid-embryogenesis, with cardiac and neural tube defects.