Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 321, Issue 1-2, Pages 123-133Publisher
SPRINGER
DOI: 10.1007/s11010-008-9926-y
Keywords
Non-transferrin iron transport; DMT1; Dcytb; Ferroportin; Hephaestin; Ceruloplasmin
Categories
Funding
- Third Faculty of Medicine of Charles University in Prague [MSM0021620814]
Ask authors/readers for more resources
We studied the effect of iron deficiency, i.e., 24-h preincubation in iron-free medium, and the effect of high level of non-transferrin iron, i.e., the preincubation in ferric citrate medium containing 500 mu M ferric citrate, on the expression of DMT1, Dcytb, ferroportin, hephaestin, and ceruloplasmin in various functional types of human cells. The expression of these proteins potentially involved in non-transferrin iron transport across cell membranes was tested on mRNA level by quantitative real-time PCR as well as on protein level by western blot analysis in Caco-2 (colorectal carcinoma), K562 (erythroleukemia), and HEP-G2 (hepatocellular carcinoma) cells. We found that changes in non-transferrin iron availability, i.e., iron deficiency and high level of non-transferrin iron, affect the expression of tested proteins in a cell type-specific manner. We also demonstrated that changes in the expression on mRNA level do not often correlate with relevant changes on protein level.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available