Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 190, Issue 1, Pages 16-22Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2013.05.007
Keywords
Trypanosoma; Choline; Transport; Phosphatidylcholine; Kinetics
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Funding
- Swiss National Science Foundation [CRSII3_127300, 31003A-130815]
- Swiss National Science Foundation (SNF) [31003A_130815, CRSII3_127300] Funding Source: Swiss National Science Foundation (SNF)
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Choline is an essential nutrient for eukaryotic cells, where it is used as precursor for the synthesis of choline-containing phospholipids, such as phosphatidylcholine (PC). According to published data, Trypanosoma brucei parasites are unable to take up choline from the environment but instead use lysophosphatidylcholine as precursor for choline lipid synthesis. We now show that T. brucei procyclic forms in culture readily incorporate [H-3]-labeled choline into PC, indicating that trypanosomes express a transporter for choline at the plasma membrane. Characterization of the transport system in T. brucei procyclic and bloodstream forms shows that uptake of choline is independent of sodium and potassium ions and occurs with a K-m in the low micromolar range. In addition, we demonstrate that choline uptake can be blocked by the known choline transport inhibitor, hemicholinium-3, and by synthetic choline analogs that have been established as anti-malarials. Together, our results show that T. brucei parasites express an uptake system for choline and that exogenous choline is used for PC synthesis. (C) 2013 Elsevier B.V. All rights reserved.
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