Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 179, Issue 2, Pages 91-99Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2011.06.004
Keywords
Proteomics; Antimony; Drug resistance; L donovani
Categories
Funding
- Department of Biotechnology, Government of India
- Council for Scientific and Industrial Research, India
- University Grants Commission, Government of India
Ask authors/readers for more resources
Visceral Leishmaniasis (VL) is a parasitic disease caused by the protozoan parasite Leishmania donovani. Resistance to pentavalent antimonials (SbV), the mainstay therapy for leishmaniasis is now a major concern, due to emergence of drug resistance. Hence, understanding the underlying mechanism of resistance to antimonials is required. Here we used quantitative mass spectrometery to identify global proteome differences between antimony-susceptible/-resistant isolates. We detected modification of expression of proteins involved in the key metabolic pathways. Comparative proteomic analysis indicated increase in glycolysis in the antimony-resistant isolates. Elevated expression of stress related proteins implicated in oxidative stress was observed in the resistant parasites. Most importantly, we observed upregulation of proteins that may have a role in intracellular survival of the parasite in the resistant isolates. The identified parasite proteins could serve as surrogate markers for resistance or susceptibility and would also help in understanding the underlying mechanism of resistance to antimonials. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available