Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 170, Issue 2, Pages 105-107Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2009.11.007
Keywords
Haemonchus contortus; Levamisole resistance; cDNA-AFLP; Molecular marker; Nicotinic acetylcholine receptor subunit; Parasite nematode
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Funding
- Region Centre
- Animal Health Division of INRA
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The cDNA-AFLP (cDNA-amplified fragment length polymorphism) method comparing transcripts from levamisole-resistant and susceptible Haemonchus contortus isolates has led to the successful identification of a number of potentially useful levamisole-resistance markers. In the present study, we report the characterization of the transcript-derived fragment (TDF) named HAX which was confirmed to be specifically expressed in three levamisole-resistant isolates by RT-PCR experiments. Cloning and sequencing of the full-length cDNA sequence of HAX revealed high similarity to the Caenorhabditis elegans acr-8 gene and its Putative H. contortus orthologue encoding a nicotinic acetylcholine receptor subunit. This Hco-acr-8b short isoform corresponded to a spliced variant of Hco-acr-8 mRNA containing the two first exons and a part of intron 2. As nicotinic acetylcholine receptors constitute the pharmacological target of levamisole, Hco-acr-8b may potentially be involved in the molecular mechanisms leading to levamisole-resistance acquisition in H. contortus. (C) 2009 Elsevier B.V. All rights reserved.
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