Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 167, Issue 2, Pages 104-117Publisher
ELSEVIER
DOI: 10.1016/j.molbiopara.2009.05.004
Keywords
Trypanosoma; Leishmania; Mitochondrial carrier family (MCF); Mitochondrion
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Funding
- Deutsche Forschungsgemeinschaft (DFG) [VO965/1-1]
- BBSRC [BB/G00448X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G00448X/1] Funding Source: researchfish
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The mitochondrial carrier family (MCF) is a group of structurally conserved proteins that mediate the transport of a wide range of metabolic intermediates across the mitochondrial inner membrane. In this paper, an overview of the mitochondrial carrier proteins (MCPs) of the early-branching kinetoplastid parasite Trypanosoma brucei brucei is presented. Sequence analysis and phylogenetic reconstruction gave insight into the evolution and conservation of the 24 identified TbMCPs; for most of these, putative transport functions could be predicted. Comparison of the kinetoplastid MCP inventory to those previously reported for other eukaryotes revealed remarkable deviations: T b. brucei lacks genes encoding some prototypical MCF members, such as the citrate carrier and uncoupling proteins. The in vivo expression of the identified TbMCPs in the two replicating life-cycle forms of T b. brucei, the bloodstream-form and procyclic-form, was quantitatively assessed at the mRNA level by Northern blot analysis. Immunolocalisation studies confirmed that majority of the 24 identified TbMCPs is found in the mitochondrion of procyclic-form T b. brucei. (C) 2009 Elsevier B.V. All rights reserved.
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