Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 165, Issue 2, Pages 142-152Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2009.01.014
Keywords
Leishmania; Life cycle; Maxicircle; Divergent region; Heteroplasmy
Categories
Funding
- Russian Foundation for Basic Research [05-04-48895a]
- International Association for the promotion of co-operation with scientists from the New Independent States of the former Soviet Union (INTAS) [2001-216, 05-1000008-8043]
Ask authors/readers for more resources
The kinetoplast genome contains several thousands of minicircles of various sequence classes and several scores of maxicircles. We demonstrated that maxicircles are heterogeneous in clonal cultures of Leishmania major, and, therefore, probably heterogeneous (heteroplasmic) within the kinetoplast. Sequence heterogeneity was observed in a non-coding fragment upstream of the 12S rRNA gene. We identified about 20 stable variants of this fragment, which were composed of one to five non-identical repeats 200-300 bp in length. Promastigote-to-amastigote and amastigote-to-promastigote differentiation was often accompanied by shifts in abundance of some maxicircle classes. Reversion to promastigote-specific maxicircle patterns was usually observed in the life cycle (promastigote-amastigote-promastigote), however there were many exceptions. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available