Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 163, Issue 2, Pages 95-106Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2008.10.005
Keywords
Excretory-secretory products; Fasciola hepatica; Apoptosis; Eosinaphil; Hydrogen peroxide; Mitochondrial depolarization
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Funding
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- Secretaria de Ciencia y Tecnologia de la Universidad Nacional de Cordoba (SECyT-UNC)
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Eosinophils (Eo) are typically associated with immune response to helminth. Previously, we demonstrated that excretory-secretory products (ESP) from Fasciola hepatica induce eosinophil apoptosis by a caspase-dependent mechanism. in this study, we observed that ESP caused mitochondrial-membrane depolarization of eosinophils leading to the release of cytochrome c. Also, ESP induced an increase in the reactive oxygen species (ROS) production, which preceded the mitochondrial injury. We found a significant rise in hydrogen peroxide, but not in the anion superoxide levels. Furthermore, catalase, but not superoxide dismutase, inhibited the mitochondrial depolarization as well as apoptosis. So, ESP induce in Eo an early increase in the ROS production, mainly hydrogen peroxide, which precedes mitochondrial injury and leads again to apoptosis. Finally, we demonstrated the participation of hydrogen peroxide in the peritoneal Eo apoptosis in vivo, both during the early stages of experimental fasciolosis in rats and after intraperitoneal ESP treatment. (C) 2008 Elsevier B.V. All rights reserved.
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