Journal
MOLECULAR & CELLULAR PROTEOMICS
Volume 11, Issue 9, Pages 800-813Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.M111.016469
Keywords
-
Categories
Funding
- Spanish Ministry of Science [CSD 2007-00020, SAF 2008-03736, BIO2009-07990, SAF 2009-07520, PS09/00101]
- Madrid regional government [CAM BIO/0194/2006]
- Fundacion Ramon Areces
- Red Tematica de Investigacion Cooperativa en Enfermedades Cardiovasculares (RECAVA, Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Ministry of Health) [RD06/0014/0030, RD06/0014/0025]
- Madrid regional government
- European Social Fund
- Instituto de Salud Carlos III
Ask authors/readers for more resources
Nitroxidative stress in cells occurs mainly through the action of reactive nitrogen and oxygen species (RNOS) on protein thiol groups. Reactive nitrogen and oxygen species-mediated protein modifications are associated with pathophysiological states, but can also convey physiological signals. Identification of Cys residues that are modified by oxidative stimuli still poses technical challenges and these changes have never been statistically analyzed from a proteome-wide perspective. Here we show that GELSILOX, a method that combines a robust proteomics protocol with a new computational approach that analyzes variance at the peptide level, allows a simultaneous analysis of dynamic alterations in the redox state of Cys sites and of protein abundance. GELSILOX permits the characterization of the major endothelial redox targets of hydrogen peroxide in endothelial cells and reveals that hypoxia induces a significant increase in the status of oxidized thiols. GELSILOX also detected thiols that are redox-modified by ischemia-reperfusion in heart mitochondria and demonstrated that these alterations are abolished in ischemia-preconditioned animals. Molecular & Cellular Proteomics 11: 10.1074/mcp.M111.016469, 800-813, 2012.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available