Journal
MOLECULAR & CELLULAR PROTEOMICS
Volume 9, Issue 7, Pages 1423-1436Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.M900449-MCP200
Keywords
-
Categories
Funding
- Direccion General de Investigacion, Subdireccion General de Proyectos de Investigacion, Ministerio de Educacion y Ciencia, Spain [BIO2007-66313]
- Ministerio de Ciencia e Innovacion, Spain
- Federation of European Microbiological Societies
- Danish National Research Foundation
- Danish Research Agency
- Lundbeck Foundation
Ask authors/readers for more resources
Streptomyces species produce many clinically important secondary metabolites, including antibiotics and antitumorals. They have a complex developmental cycle, including programmed cell death phenomena, that makes this bacterium a multicellular prokaryotic model. There are two differentiated mycelial stages: an early compartmentalized vegetative mycelium (first mycelium) and a multinucleated reproductive mycelium (second mycelium) arising after programmed cell death processes. In the present study, we made a detailed proteomics analysis of the distinct developmental stages of solid confluent Streptomyces coelicolor cultures using iTRAQ (isobaric tags for relative and absolute quantitation) labeling and LC-MS/MS. A new experimental approach was developed to obtain homogeneous samples at each developmental stage (temporal protein analysis) and also to obtain membrane and cytosolic protein fractions (spatial protein analysis). A total of 345 proteins were quantified in two biological replicates. Comparative bioinformatics analyses revealed the switch from primary to secondary metabolism between the initial compartmentalized mycelium and the multinucleated hyphae. Molecular & Cellular Proteomics 9:1423-1436, 2010.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available