Journal
MOLECULAR & CELLULAR PROTEOMICS
Volume 9, Issue 6, Pages 1157-1166Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.M900426-MCP200
Keywords
-
Categories
Funding
- Max-Planck-Society
- Deutsche Forschungsgemeinschaft [Br1416]
- European Union Commission
- Kerckhoff Foundation
- Excellence Initiative Cardiopulmonary System
Ask authors/readers for more resources
Despite progress in the characterization of their genomes, proteomes of several model organisms are often only poorly characterized. This problem is aggravated by the presence of large numbers of expressed sequence tag clones that lack homologues in other species, which makes it difficult to identify new proteins irrespective of whether such molecules are involved in species-specific biological processes. We have used a pulsed stable isotope labeling with amino acids in cell culture (SILAC)-based mass spectrometry method, which is based on the detection of paired peptides after [C-13(6)]lysine incorporation into proteins in vivo, to greatly increase the confidence of protein identification in cross-species database searches. The method was applied to identify nearly 3000 proteins in regenerating tails of the urodele amphibian Notophthalmus viridescens, which possesses outstanding capabilities in the regeneration of complex tissues. We reason that pulsed in vivo SILAC represents a versatile tool to identify new proteins in species for which only limited sequence information exists. Molecular & Cellular Proteomics 9:1157-1166, 2010.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available