Journal
MITOCHONDRION
Volume 19, Issue -, Pages 144-153Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2014.05.003
Keywords
Protein interaction; Regulation; Structure; BiFC; Transmission Electron Microscopy
Categories
Funding
- NSF [IOB-0325656]
- Missouri Agricultural Experiment Station
- University of Missouri Food for the 21st Century Program
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The Arabidopsis thaliana genome includes three genes for mitochondrial dihydrolipoamide acetyltransferase, the E2-component of the mitochondrial pyruvate dehydrogenase complex (PDC). Two genes encode E2-proteins with a single lipoyl domain, while the third has a two-lipoyl domain structure. Transcripts for each E2 protein were expressed in all plant organs. Each recombinant AtmtE2 can individually form an icosahedral PDC core structure, and results from bimolecular fluorescence complementation assays are consistent with formation of hetero-core structures from all permutations of the AtmtE2 proteins. We propose a unique regulatory mechanism involving dynamic formation of hetero-core complexes that include both mono- and di-lipoyl forms of AtmtE2. (C) 2014 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
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