Journal
MITOCHONDRION
Volume 14, Issue -, Pages 54-63Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2013.11.003
Keywords
Mitochondrial biogenesis; Glycogen synthase kinase-3; 2-Deoxyglucose
Categories
Funding
- Chulabhorn Research Institute
- Center of Excellence on Environmental Health and Toxicology (EHT), Office of the Higher Education Commission, Thailand
Ask authors/readers for more resources
Mitochondrial biogenesis, a mitochondrial growth and division process, is crucial for adaptation to metabolic stress. The present study demonstrated that treatment with a specific inhibitor of GSK3, SB216763, attenuated induction of mitochondrial biogenesis by a glycolysis inhibitor, 2-deoxyglucose (2-DG), without affecting this biogenesis at basal condition. Additionally, overexpression of WT-GSK3 beta promoted whereas GSK3 beta-KD attenuated 2-DG-induced mitochondrial protein expression. The mitochondrial biogenesis attenuation by GSK3 inhibitor was not due to inhibition of protein degradation. Furthermore, GSK3 inhibition further reduced transcription of mitochondrial (COXII), but not nuclear (VDAC) gene by 2-DG suggesting its participation in 2-DG-induced mitochondria! transcription. Together, our results show that GSK3 regulates mitochondrial biogenesis induced by glycolysis inhibition. (C) 2013 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available