Journal
MITOCHONDRION
Volume 13, Issue 1, Pages 7-14Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2012.11.001
Keywords
Human; Platelets; Mitochondria; OXPHOS; High-resolution respirometry; Mitochondrial disorders
Categories
Funding
- Swedish Research Council [2011-3470]
- Royal Physiographic Society
- Foundation of the Swedish National Board of Health and Welfare
- Carl og Ellen Hertz' legat til Dansk laege- og naturvidenskab
- Lippman foundation
- Grants-in-Aid for Scientific Research [23592266] Funding Source: KAKEN
Ask authors/readers for more resources
Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondria] physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondria] respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability. (c) 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available