Journal
MITOCHONDRION
Volume 12, Issue 3, Pages 391-398Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2012.02.004
Keywords
OXPHOS; mRNA localization; Mitochondria-bound polysomes; YB-1; Translation
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [19209019, 21590337]
- Grants-in-Aid for Scientific Research [21590337, 24659281, 24590387, 19209019] Funding Source: KAKEN
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The mitochondrial oxidative phosphorylation (OXPHOS) proteins are encoded by both nuclear and mitochondrial DNA. The nuclear-encoded OXPHOS mRNAs have specific subcellular localizations, but little is known about which localize near mitochondria. Here, we compared mRNAs in mitochondria-bound polysome fractions with those in cytosolic, free polysome fractions. mRNAs encoding hydrophobic OXPHOS proteins, which insert into the inner membrane, were localized near mitochondria. Conversely, OXPHOS gene which mRNAs were predominantly localized in cytosol had less than one transmembrane domain. The RNA-binding protein Y-box binding protein-1 is localized at the mitochondrial outer membrane and bound to the OXPHOS mRNAs. Our findings offer new insight into mitochondrial co-translational import in human cells. (C) 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
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