Journal
MITOCHONDRION
Volume 12, Issue 1, Pages 126-131Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2011.05.010
Keywords
Mitochondrial NOS (mtNOS); Nitric oxide (NO); Oxygen consumption; Oxygen kinetics; Respiration; Skeletal muscle
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Funding
- Spanish Ministry of Science and Innovation (MICINN) [BFI2003-03493]
- Instituto de Salud Carlos III [FIS PI060701, PS09/00116, CP08/00204]
- MICINN
- 'Ramon y Cajal' program
- 'Miguel Servet' program
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The possible existence of a mitochondrially localized nitric oxide (NO) synthase (mtNOS) is controversial. To clarify this, we studied the ability of intact mitochondria to generate NO and the effect of mitochondrial NO on respiration. Respiratory rates and oxygen kinetics (P-50 values) were determined by high-resolution respirometry in skeletal-muscle mitochondria from control mice and mice injected with Escherichia coli lipopolysaccharide (LPS). In the presence of the NOS substrate L-arginine, mitochondria from LPS-treated mice had lower respiration rates and higher P-50 values than control animals. These effects were prevented by the NOS inhibitor L-NMMA. Our results suggest that mitochondrially derived NO is generated by an LPS-inducible NOS protein other than iNOS and modulates oxygen consumption in mouse skeletal muscle. (C) 2011 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
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