Journal
MICROSCOPY RESEARCH AND TECHNIQUE
Volume 75, Issue 3, Pages 374-378Publisher
WILEY
DOI: 10.1002/jemt.21066
Keywords
agouti; blood cells; morphology; morphometry; mensuration
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Thirty adult agoutis (Dasyprocta primnolopha) from the Nucleus of Study and Preservation of Wild Animals at the Federal University of Piaui were used. Blood scrubs of these animals were colored by the Leishman method and analyzed in light microscopy. The cells had been measured using programs that analyze images (Leica QWin Image Processing and Analysis Software). Mature erythrocytes, basophil reticulocytes, lymphocytes, eosinophils, neutrophils, monocytes, and thrombocytes were identified. Agoutis' erythrocytes presented elliptical form, without nucleus with an average diameter of 5.64 micromeres +/- 0.38. The lymphocytes are spherical cells with scarce cytoplasm, dense and with a very centralized rounded nucleus measuring an average diameter of 13.20 micromeres +/- 0.35. The monocytes are slightly basophilic, with a spherical nucleus, central constriction, and an average diameter of 20.59 micromeres +/- 0.32. The neutrophils are spherical, with a polymorphic lobulated nucleus, with an average diameter of 11.2 micromeres +/- 0.20. The eosinophils are spherical with lobulated nucleus and with an average diameter of 14.25 micromeres +/- 0.36. Only five basophils were observed, with abundance of cytoplasmic granules with 9.8 micrometers of diameter +/- 0.30. Thrombocytopenic pleomorphism was frequent. There were similarities in the cellular constituents in peripheral blood of agoutis and of other rodents and humans. The cellular types from the peripheral blood, the morphology, and morphometry of the blood's cells did not vary according to sex. (c) Microsc. Res. Tech. 75:374-378, (c) 2011 Wiley Periodicals, Inc.
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