Journal
MICROSCOPY AND MICROANALYSIS
Volume 17, Issue 2, Pages 215-219Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S1431927610094201
Keywords
nano-CT; X-ray tomography; corrosion casts; computer modeling
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Funding
- The Fund for Scientific Research-Flanders (FWO) [G.0100.08]
- Ghent University [CRA.01G01008]
- EPSCoR
- Office Of The Director [814251] Funding Source: National Science Foundation
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A vascular corrosion cast of an entire mouse kidney was scanned with a modular multiresolution X-ray nanotomography system. Using an isotropic voxel pitch of 0.5 mu m, capillary systems such as the vasa recta, peritubular capillaries and glomeruli were clearly resolved. This represents a considerable improvement over corrosion casts scanned with microcomputed tomography systems. The resolving power of this system was clearly demonstrated by the unique observation of a dense, subcapsular mat of capillaries enveloping the entire outer surface of the cortical region. Resolution of glomerular capillaries was comparable to similar models derived from laser scanning confocal microscopy. The high-resolution, large field of view and the three-dimensional nature of the resulting data opens new possibilities for the use of corrosion casting in research.
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