Journal
MICROPOROUS AND MESOPOROUS MATERIALS
Volume 143, Issue 2-3, Pages 341-347Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micromeso.2011.03.017
Keywords
Carboxyl-modified SBA-15; Papain; H2SO4 treatment; Immobilization ability; Activity
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Funding
- National Natural Science Foundation of China [20773069]
- Natural Science Foundation of Tianjin [08JCZDJC21400]
- Fundamental Research Funds for the Central Universities
- MOE [IRT-0927]
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Carboxyl-modified mesoporous materials have been demonstrated as efficient carriers for immobilizing enzymes. However, little attention has been paid to the effect of H2SO4 treatment, a key in the synthesis of carboxyl-modified carriers, on the immobilization. To examine the H2SO4 treatment's influence, we contrasted the ability of immobilizing papain between SBA-15 and the one after H2SO4 treatment (H2SO4-treated SBA-15). Meanwhile, the contribution of the as-synthesized carboxyl to the immobilization was also rechecked by comparing carboxyl-modified SBA-15 (COOH-SBA-15) and H2SO4-treated SBA-15. In relation to the experiments, the H2SO4 treatment increases the immobilizing ability of SBA-15, especially when the immobilization performs near the pl of papain. Moreover, the as-synthesized carboxyl facilitates the immobilization by means of strengthening the hydrophobic dehydration at the interface, instead of merely through electrostatic forces. Additionally, the carboxyl makes a large contribution to inducing the favorable conformation change of papain, improving the specific activity, catalytic efficiency, and binding affinity of the immobilized enzyme when combined with the substrate. (C) 2011 Elsevier Inc. All rights reserved.
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