Journal
MICROPOROUS AND MESOPOROUS MATERIALS
Volume 114, Issue 1-3, Pages 507-510Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micromeso.2007.12.027
Keywords
penicillin G acylase (PGA); immobilization; mesoporous silica; pore size; mesophase
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Penicillin G acylase (PGA) was immobilized on KIT-6 and SBA-15 with different pore sizes to study the influence of pore size, pore volume and mesophases on the immobilized enzyme (IME) activity. The results show that the pore size, pore volume, mesophase and surface area have,in obvious influence on the IME activity, of which the pore size is the most important factor. The activity of PGA immobilized on KIT-6/130 reaches to 3522 IU/g by dry support, which is higher than that ever reported for carrier-bound penicillin G acylase [A.I. Kallenberg, F. van Rantwijk, R.A. Sheldon, Adv. Synth. Catal. 347 (2005) 905-926]. Because of the covalent immobilization, the operational stability of IME increases. (C) 2007 Elsevier Inc. All rights reserved.
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