Journal
MICRON
Volume 48, Issue -, Pages 26-33Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micron.2013.02.003
Keywords
Atomic force microscopy; Quantitative imaging; Microorganisms; Eukaryotic cells; Imaging; Nanomechanical properties
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Funding
- Direction Generale de l'Armement (DGA)
- ANR Young Scientist Program AFMyst [30024332]
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Since the last 10 years, AFM has become a powerful tool to study biological samples. However, the classical modes offered (imaging or tapping mode) often damage sample that are too soft or loosely immobilized. If imaging and mechanical properties are required, it requests long recording time as two different experiments must be conducted independently. In this study we compare the new QI (TM) mode against contact imaging mode and force volume mode, and we point out its benefit in the new challenges in biology on six different models: Escherichia coli, Candida albicans, Aspergillus fumigatus, Chinese hamster ovary cells and their isolated nuclei, and human colorectal tumor cells. (C) 2013 Elsevier Ltd. All rights reserved.
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