4.5 Article

Analysis of sperm concentration and motility in a microfluidic device

Journal

MICROFLUIDICS AND NANOFLUIDICS
Volume 10, Issue 1, Pages 59-67

Publisher

SPRINGER HEIDELBERG
DOI: 10.1007/s10404-010-0646-8

Keywords

Microfluidics; Sperm; Infertility; Resistive pulse technique

Funding

  1. National Science Council [NSC98-2120-M-002-009, NSC94-2212-E-002-054]

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A home-use device that allows rapid and quantitative sperm quality analysis is desirable but not yet fully realized. To aid this effort, this article presents a microfluidic device capable of quantifying sperm quality in terms of two critical fertility-related parameters-motile sperm concentration and motility. The microdevice produces flow field for sperms to swim against, and sperms that overcame the flow within a specified time are propelled along in a separate channel and counted via resistive pulse technique. Data are compared to two control methods clinically utilized for sperm quality exam-hemocytometer and the sperm quality analyzer. Results reveal the numbers of pulses generated by passage of sperms correlates strongly with the two control methods: pulse number from 0 to 335 corresponds to progressively motile sperm concentrations from 0 to 19 x 10(6)/ml (hemocytometer) and Sperm Motility Index from 0 to 204 (sperm quality analyzer). The microdevice should be applicable to facilitate self-assessment of sperm quality at home.

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