Journal
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
Volume 51, Issue 5, Pages 548-555Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/00365521.2015.1117650
Keywords
Biological markers; Crohn disease; enzyme-linked immunosorbent assay; faeces; inflammatory bowel diseases; leukocyte L1 antigen complex; ulcerative colitis
Categories
Funding
- AbbVie Inc.
Ask authors/readers for more resources
Objective Faecal (f-) calprotectin is a biomarker of intestinal inflammation. Previous studies have described intra-individual day-to-day variability of this biomarker in patients with inflammatory bowel disease (IBD) and morning samples have been suggested for standardisation purposes. With this project, we investigated if day-to-day variability differed from diurnal variability. Additionally, we evaluated a new extraction method for f-calprotectin analysis. Methods Fifty patients provided three faeces samples from morning - evening - morning on two consecutive days. Nineteen patients provided two faeces samples from the same bowel movement, one conventional spot sample, and one sample with a device for patient-administered sampling and extraction. Results The two morning samples differentiated between mucosal inflammation and mucosal healing with same level of agreement as the two samples from the same day (kappa 0.76), using an f-calprotectin cut-off level of 259g/g. Although large intra-individual variation in f-calprotectin values, there were no significant day-to-day (p=0.096) or diurnal variation (p=0.78). Used by laboratory technicians, the new extraction device correlated significantly with the conventional extraction method (p<0.001), Spearman's rank correlation coefficient 0.95. Of the 19 patients testing patient administered extraction, two patients provided samples leading to considerably higher f-calprotectin levels than conventional sampling procedure. Conclusions The reliability of f-calprotectin morning samples is equal to the reliability of samples from different bowel movements on the same day. The new extraction method is reliable when used by laboratory technicians, but larger studies are recommended to evaluate patient administered extraction.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available