Journal
MICROELECTRONIC ENGINEERING
Volume 110, Issue -, Pages 70-74Publisher
ELSEVIER
DOI: 10.1016/j.mee.2013.01.053
Keywords
Gelatin; Gel-embossing; Cell culture; Nanopatterns
Categories
Funding
- European Commission [IRSES-GA-2009-247641 (Microcare)]
- French National Research Agency [ANR-12-RPIB-0015]
- NSF of China [11174012]
- [24651136]
- [22350104]
- Agence Nationale de la Recherche (ANR) [ANR-12-RPIB-0015] Funding Source: Agence Nationale de la Recherche (ANR)
- Grants-in-Aid for Scientific Research [23681028, 22350104, 24651136] Funding Source: KAKEN
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We developed a gel-embossing technique which could be used to fabricate' gelatin nanopatterns for cell culture studies. A gel of gelatin solution was dropped on the surface of a glass slide and embossed using a gas permeable stamp made of polydimethylsiloxane (PDMS). After drying in a desiccator, nanostructure patterns could be replicated from the stamp into the gelatin layer. After crosslinking in a 10% formaldehyde solution in phosphate buffered saline (PBS), the embossed gelatin layer could be used for cell culture studies. The gel-embossed patterns before and after crosslinking were characterized by atomic force microscopy, revealing significant changes of the pattern morphology introduced by both gel-emboss and crosslink processes. Nonetheless, strong effedts of cell elongation and fast cell growth were observed for the proof-of-concept using NIH-3T3 cells cultured on gelatin nanogrooves. (C) 2013 Elsevier B.V. All rights reserved.
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