Journal
MICROELECTRONIC ENGINEERING
Volume 88, Issue 8, Pages 1693-1697Publisher
ELSEVIER
DOI: 10.1016/j.mee.2011.01.047
Keywords
Microfluidic; Cell culture; Extra cellular matrix
Categories
Funding
- European Projects [CP-FP 214566-2 (Nanoscales), IRSES-GA-2009-247641 (Microcare)]
- [22710116]
- Grants-in-Aid for Scientific Research [22710116, 22350104] Funding Source: KAKEN
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Microfluidic devices with bi-layer substrates of different stiffness but the same surface chemistry were used for cell culture studies. Whereas the proposed microfluidic culture platform is advantageous for data collection and minimum consumption of material and reagents, the bi-layer substrate provides unique alternation of material properties to elucidate the mechanic responses of different types of cells, including mouse embryo fibroblasts, embryonic stem cells and cerebella granule cells. (C) 2011 Elsevier B.V. All rights reserved.
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