Journal
MICROCIRCULATION
Volume 20, Issue 7, Pages 617-628Publisher
WILEY
DOI: 10.1111/micc.12061
Keywords
toll-like receptor 4; nuclear factor kappa B p65; activator protein-1; adhesion molecules; Rheum palmatum
Categories
Funding
- Ministry of Science and Technology of the People's Republic of China [2008ZX09401]
Ask authors/readers for more resources
ObjectiveSepsis is a systemic inflammatory response syndrome. Emodin is a major ingredient of Rheum Palmatum, a Chinese herb that is widely used in China for treatment of endotoxemia-related diseases. This study intended to examine the effect of Emodin on LPS-induced rat mesenteric microcirculatory disturbance and the underlying mechanisms. MethodsThe male Wistar rats received LPS (5 mg/kg/hr) for 90 min, with or without administration of Emodin (10 mg/kg/hr) by enema 30 min before (pre-treatment) or after (post-treatment) LPS infusion, and the dynamics of mesenteric microcirculation were determined by inverted intravital microscopy. Expression of adhesion molecules and TLR4, NF-B p65, ICAM-1, MPO, and AP-1 in mesentery tissue was evaluated by flow cytometry and Western-blot, respectively. ResultsPre or post-treatment with Emodin significantly ameliorated LPS-induced leukocyte emigration, reactive oxygen species production and albumin leakage, and the expression of TLR4, NF-B p65, ICAM-1, MPO and AP-1 in mesentery. ConclusionsThese results demonstrate the beneficial role of Emodin in attenuating the LPS-induced microcirculatory disturbance, and support the use of Emodin for patients with endotoxemia.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available