Journal
MICROCHIMICA ACTA
Volume 185, Issue 10, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-2990-4
Keywords
AuNPs; Split DNAzyme; Amplification; Enzyme-freedetection; T-Hg(II)-T
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Funding
- Chongqing Key Laboratory of Catalysis and New Environmental Materials [KFJJ2017033]
- Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences [201712]
- Science and Technology Research Program of Chongqing Municipal Education Commission [KJ1706156]
- Project of Wenzhou Science & Technology Bureau [W20170006]
- State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials [17kffk06]
- Startup Foundation of Chongqing Technology and Business University [1756001]
- National Natural Science Foundation of China [21601164, 31300819]
- [CQCM-2016-05]
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A catalytic cleavage strategy was developed for the fluorometric determination of Hg(II). The method is based on the use of a Mg(II)-dependent split DNAzyme. Fluorophore labeled hairpins were conjugated to gold nanoparticles (AuNPs) upon which fluorescence is quenched. Thymine-Hg(II)-thymine (T-Hg(II)-T) interaction causes the two DNA sequences to form an entire enzyme-strand DNA (E-DNA). The E-DNA bind to the hairpins on the AuNPs to form a Mg(II)-dependent DNAzyme structure. The circular cleavage of hairpins results in a signal amplification and in the recovery of fluorescence. The assay has a limit of detection (LOD) as low as 80 pM of Hg(II). This LOD is comparable to those obtained with other amplification strategies. The method was successfully applied to the determination of Hg(II) in Chinese herbs (Atractylodes macrocephala Koidz).
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